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My last westerns?

I’ve written before about western blots (I’ve written an overview here), but as I was running what may become my last protein gel, I thought I’d just document it and write a lengthier post on them. So as I’ve written before, we use western blots to visualise proteins. I’m currently […]

Nearly submission time…

One week left until the submission deadline for my Ph. D thesis! Thankfully I’m at the stage now where I’m just looking for spelling/grammatical/punctuation errors. All the major comments from both of my supervisors have been addressed, all my figures have been finalised… It’s a good place to be. So […]

General update

Where did February go?? January wasn’t too bad but February has been intense. Since my last official update, I’ve progressed through my cloning woes to get two of my plasmids, plasmid #8 and #9. Plasmid #2 is being a pain, so I haven’t got the green insert in yet. But- […]

I’m still alive!

It’s been over two weeks since my last post, so I thought I’ll just write a short update just to say I’m still alive and trying to finish up with my thesis and such. I’ll post a longer update on the weekend! I also started trying to jog, as a […]

No, not the beer

2020 has seriously been a weird year so far! First it’s been bushfires, and now it’s this weird, novel Coronavirus (2019-nCoV)… Aside from political issues, the world is seriously falling apart (*coughs ‘also climate change’*)! The institute I work at has recently been featured heavily on the news, as one […]

The clone woes (part 2)

Part 2 of the clone woes (as I’ve dubbed it) is the current update on what’s happening. Just as a reminder from part 1, I’m currently trying to clone three different plasmids. Due to some technical issues involving the restriction enzyme EcoRI, I’ve had to revise my strategy. I got […]

Writing a Ph. D thesis

Ah, yes. The Ph. D thesis. It’s that thing that you have to write and submit, in order to be eligible to graduate and become a Doctor (but not the medical kind). I’ve spoken to quite a few people over the years about writing a thesis. The general consensus has […]

Visualising DNA

So I posted the above meme on Twitter, and it got some good laughs. But while I was cutting my DNA bands after my DNA digestion (please see my previous post on this, but it’s step 5 of the cloning stage), it occurred to me that I could take a […]

The clone woes

This piece is filled with cloning jargon! You have been warned! In a previous post, I explained my upcoming experiments in a bit of detail. Prepare for an update on how that’s all going! FYI highly recommend reading the previous post to make sense of this current one. I’m currently […]